Advances In Native Binding Assays

Isothermal titration calorimetry (ITC) is a technique where heat is measured under constant pressure. This heat can be translated into enthalpy based on the First Law of Thermodynamics. Although the focus of the technique has been rooted in measuring biophysical phenomena in labs that specialized in calorimetry, it has seen growth as a general technique to quantitatively describe systems from proteins to nanomaterials. This affinity assay is a native test that doesn’t rely on intrinsic or extrinsic fluorphores like other binding techniques, and neither of the binding partners are immobilized onto a chip or probe. Additionally, ITC provides more than affinity; all the thermodynamic properties as well as stoichiometry of chemical or physical equilibria are measured in a single experiment. Explore advances that will enable investigators to expand their research using ITC.