AI-Enabled Phenotypic Analysis Of Label-Free Intestinal Organoids

By Zhisong Tong and Prathyushakrishna Macha, Molecular Devices

This content is brought to you by Molecular Devices, a Danaher Operating Company.

Three-dimensional (3D) models, including spheroids and organoids, are becoming increasingly popular in various research fields due to their ability to more accurately mimic the in vivo environment compared to traditional two-dimensional (2D) monolayer culture models. These 3D models provide a more realistic representation of tissue architecture and cellular interactions, which are crucial for understanding complex biological processes. Recent advancements in organoid culture techniques have enabled scientists to cultivate organ-like structures in 3D using either induced pluripotent stem cells (iPSCs) or adult stem cells.

In this application note, we outline comprehensive approaches for utilizing imaging and image analysis techniques to describe and quantify changes in phenotypes and the morphology of organoids. These methods are versatile and can be applied to any type of organoid, regardless of their origin or the protocols used for their development, and allow for a straightforward method to image, analyze, and classify intestinal organoids into three distinct categories: immature, intermediate, and mature. The AI-enabled classification technique is particularly valuable for monitoring the growth and development of intestinal organoids in culture.

Access the full application note to learn more.