Application Of LC-UV/MS Workflows To Increase Efficiency In Impurity Profiling Of GLP-1 Analogs

By Duanduan Han, Samantha Ippoliti, Robert E. Birdsall, and Karen Nyholm, Waters Corporation

Glucagon-like peptide-1 (GLP-1) receptor agonists have recently emerged as powerful metabolic regulators in the treatment of type 2 diabetes and obesity. Their growing popularity as weight-loss therapies has not only driven up demand but also accelerated research into novel analogs and innovative delivery methods. However, this surge has introduced significant analytical challenges—ranging from peptide synthesis and impurity profiling to stability monitoring—as manufacturers strive to enhance efficiency and scale up production. In this context, LC-UV/MS platforms present a compelling solution to offer analytical laboratories the ability to streamline late-stage method development and early manufacturing processes.

This application note highlights the unique capabilities of an LC-UV/MS workflow in identifying impurities in GLP-1 analogs subjected to chemical and thermal stress, which showcases its potential to accelerate and refine analytical processes. Explore how this advanced workflow can transform your lab’s efficiency.