Assess Biomolecular Stability With Confidence Using Nano DSC

Nano DSC is a high-resolution technique used to evaluate the thermal stability of biomolecules and biotherapeutics. By directly measuring the heat absorbed during structural changes, Nano DSC provides valuable insights into protein unfolding and the melting behavior of nucleic acids and lipids. The resulting data reveal both the temperature at which these transitions occur and the total heat associated with the event, providing a comprehensive picture of molecular stability.

Designed for liquid sample analysis, the Nano DSC is a highly sensitive differential scanning calorimeter that delivers accurate, reproducible results. Experiments are controlled through intuitive software, allowing samples to be heated at a precisely defined rate across a specified temperature range. For higher throughput applications, the system can be configured with an automated sampler that draws directly from temperature-controlled 96-well plates, enabling unattended operation. Automated sample loading and cleaning help ensure consistency between runs while minimizing sample carryover.

Unlike many other stability-testing methods, Nano DSC requires no dyes, labels, or other added reagents, allowing researchers to analyze samples in their native state. This simplifies workflows, reduces the risk of experimental artifacts, and supports more reliable decision-making throughout biopharmaceutical development. Its exceptional sensitivity and reproducibility make it easy to detect changes that may occur during manufacturing, storage, or formulation development.

In a single experiment, researchers can obtain key thermodynamic parameters, including melting temperature (Tm), enthalpy (ΔH), and heat capacity change (ΔCp). These measurements can be used to calculate free energy and compare formulation stability, helping researchers identify optimal conditions for product development and long-term storage.

Watch our 3-minute video to see Nano DSC in action and learn how it can help accelerate stability studies in your lab.