When harvesting cell cultures with high density ( > 250x10^6 cells/mL) or high turbidity ( > 3000 NTU), depth filters can quickly clog and bring the entire process to a standstill. In addition to creating delays, replacing the clogged filters is messy and introduces risks of contamination along with reduced yield.
Use of unique, single-use Thermo Scientific™ CentriPAK™ BioProcess Containers (BPC) and the Thermo Scientific™ Sorvall™ BIOS 16 Centrifuge is an excellent method to pre-clarify supernatants prior to depth filtration or downstream processing. This method reduces overall processing time by eliminating pre-setup and post-cleaning steps as well as avoiding in-process clog-based delays. The sterilized, closed system ensures confidence in culture yield and purity. In this example, we harvest supernatant from a 50 L mammalian culture.