Considerations for Multiplex Live Cell Imaging And Simultaneous Multicolor Imaging For Successful Experiments

By Dr. Patric Pelzer, Leica Microsystems

Live-cell imaging experiments enable researchers to visually record cells in their living state, without disturbing artifacts that could be introduced by fixation or the termination of the various living processes. Live-cell imaging makes it possible to observe dynamic processes in cells, tissues, or whole organisms in their natural environment, rather than simply obtaining endpoint images and data.

Explore further into why it's important to use more than one fluorescent marker, considerations for image acquisition, alternatives for improving live-cell imaging, and more.