Application Note

Cultivation Of CHO Suspension Cells And SEAP Expression In The Wave-Mixed Single-Use Finesse SmartRocker Bioreactor

Source: Thermo Fisher Scientific

This application note describes the cultivation of CHO suspension cells in the wave-mixed single-use Finesse SmartRocker bioreactor with a maximum working volume of 5 L. Using a chemically defined minimal medium, it was possible to produce up to 16.4∙106 cells∙mL-1. SEAP expression was induced by medium exchange and a temperature shift. Maximum SEAP activity of up 33.1 U∙mL-1 was achieved. The results are comparable to cultivations in other wave-mixed bioreactor types. By Nadezda Perepelitsa, Stephan C. Kaiser and Dieter Eibl