Cultivation Of CHO Suspension Cells And SEAP Expression In The HyPerforma Glass Bioreactor

By Stephan C. Kaiser, Staff Scientist, Research and Development, Thermo Fisher Scientific, Santa Clara, USA; Katharina Blaschczok, Production Engineer, Roche, Basel, Switzerland; Dieter Eibl, Professor, ZHAW School of Life Sciences and Facility Management, Centre for Biochemical Engineering and Cell Cultivation Technique, Wädenswil, Switzerland

This application note describes the cultivation of CHO suspension cells in the Thermo Scientific™ HyPerforma™ Glass Bioreactor with a maximum working volume of 2.0 L. Using chemically defined minimal media, cell densities of up to 7.44 x 10⁶ cells/mL were achieved. Expression of SEAP protein was induced by medium exchange and
temperature shift. Maximum SEAP activity of 63 U/mL was reached.