Application Note

Cultivation Of CHO Suspension Cells And SEAP Expression In The HyPerforma Glass Bioreactor

By Stephan C. Kaiser, Staff Scientist, Research and Development, Thermo Fisher Scientific, Santa Clara, USA; Katharina Blaschczok, Production Engineer, Roche, Basel, Switzerland; Dieter Eibl, Professor, ZHAW School of Life Sciences and Facility Management, Centre for Biochemical Engineering and Cell Cultivation Technique, W├Ądenswil, Switzerland

Figure 1
Figure 1. The HyPerforma Glass Bioreactor, and HyPerforma G3Lab Controller with TruBio software.

This application note describes the cultivation of CHO suspension cells in the Thermo Scientific™ HyPerforma™ Glass Bioreactor with a maximum working volume of 2.0 L. Using chemically defined minimal media, cell densities of up to 7.44 x 106 cells/mL were achieved. Expression of SEAP protein was induced by medium exchange and
temperature shift. Maximum SEAP activity of 63 U/mL was reached.

 

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