Cultivation Of CHO Suspension Cells And SEAP Expression In The HyPerforma Rocker Bioreactor

By Nadezda Perepelitsa, Stephan C. Kaiser and Dieter Eibl

This application note describes the cultivation of CHO suspension cells in the wave-mixed Thermo Scientific™ HyPerforma™ Rocker Bioreactor with a maximum working volume of 5 L. Using chemically defined minimal media, cell densities of up to 16.4 x 10⁶ cells/mL were achieved. Secreted alkaline phosphatase (SEAP) expression was induced by medium exchange and temperature shift. A maximum SEAP activity of 33.1 U/mL was reached.