Cultivation Of CHO Suspension Cells And SEAP Expression In The HyPerforma Rocker Bioreactor
Source: Thermo Fisher Scientific
By Nadezda Perepelitsa, Stephan C. Kaiser and Dieter Eibl

This application note describes the cultivation of CHO suspension cells in the wave-mixed Thermo Scientific™ HyPerforma™ Rocker Bioreactor with a maximum working volume of 5 L. Using chemically defined minimal media, cell densities of up to 16.4 x 106 cells/mL were achieved. Secreted alkaline phosphatase (SEAP) expression was induced by medium exchange and temperature shift. A maximum SEAP activity of 33.1 U/mL was reached.
access the Application Note!
Log In
Get unlimited access to:
Trend and Thought Leadership Articles
Case Studies & White Papers
Extensive Product Database
Members-Only Premium Content
Welcome Back! Please Log In to Continue.
X
Enter your credentials below to log in. Not yet a member of Pharmaceutical Online? Subscribe today.
Subscribe to Pharmaceutical Online
X
Subscribe to Pharmaceutical Online
Thermo Fisher Scientific
This website uses cookies to ensure you get the best experience on our website. Learn more