By Stephan C. Kaiser, Staff Scientist, Research and Development, Thermo Fisher Scientific, Santa Clara, USA, Nadezda Perepelitsa, GMP Process Engineer, Johnson & Johnson, Basel, Switzerland
This application note describes the cultivation of Sf9 suspension cells in the Thermo Scientific™ HyPerforma™ Rocker Bioreactor with a maximum working volume of 5.0 L. Using a serum-free medium without components of animal origin, viable cell densities of up to 6.2 x 106 cells/mL were achieved. Expression of recombinant secreted alkaline phosphatase (rSEAP) was induced using a baculovirus expression vector system. rSEAP activity up to a maximum of 58.5 U/mL was achieved. The results are in the typical range observed in previous cultivations using wave-mixed bioreactor types.