Cultivation Of Sf9 Insect Cells And rSEAP Expression In The HyPerforma Rocker Bioreactor

By Stephan C. Kaiser, Staff Scientist, Research and Development, Thermo Fisher Scientific, Santa Clara, USA, Nadezda Perepelitsa, GMP Process Engineer, Johnson & Johnson, Basel, Switzerland

Summary
This application note describes the cultivation of Sf9 suspension cells in the Thermo Scientific™ HyPerforma™ Rocker Bioreactor with a maximum working volume of 5.0 L. Using a serum-free medium without components of animal origin, viable cell densities of up to 6.2 x 10⁶ cells/mL were achieved. Expression of recombinant secreted alkaline phosphatase (rSEAP) was induced using a baculovirus expression vector system. rSEAP activity up to a maximum of 58.5 U/mL was achieved. The results are in the typical range observed in previous cultivations using wave-mixed bioreactor types.