Direct And Rapid Multi-Attribute Monitoring Of Multiple Intact Monoclonal Antibodies With A Wide Isoelectric-Point Range

By Scott Mack, Zhichang Yang, Zoe Zhang, Rita Nichiporuk, and Maggie Ostrowski SCIEX, USA

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Researchers demonstrate a novel integrated workflow using the Intabio ZT system to show how it can offer direct chip-based integration of icIEF with mass spectrometry (MS), which affords confident characterization of different proteoforms of biotherapeutics and reliable quantitation of charge variants. As recombinant monoclonal antibodies (mAbs) grow in importance among biotherapeutic classes, they are proving effective in the treatment various diseases due to their high specificity and efficacy. When a platform assay using the direct chipbased integration of icIEF-UV/MS was employed to characterize 3 mAbs spanning a wide pI range it enabled a streamlined workflow to separate charge variants and offered high sensitivity and selectivity to detect low-abundant proteoforms.

Review the results of this study along with key features of the icIEF-UV/MS workflow.