Direct And Rapid Multi-Attribute Monitoring Of Intact Monoclonal Antibodies By icIEF-UV/MS

By Scott Mack, Zhichang Yang, Rashmi Madda, Jingwen Ding, Zoe Zhang, Rita Nichiporuk, and Maggie Ostrowski SCIEX, USA

Explore a novel integrated workflow using the Intabio ZT system. This innovative system offers direct chip-based integration of imaged capillary isoelectric focusing (icIEF) with mass spectrometry (MS). The streamlined workflow affords confident characterization of proteins and reliable quantitation of charge variants by icIEF-UV/MS. It also demonstrates the ability to separate charge variants by icIEF and identify them with ZenoTOF 7600 system