Direct qPCR Amplification From Human Urine Samples

This content is brought to you by Integrated DNA Technologies, a Danaher Operating Company.

Advances in qPCR are reshaping how researchers approach microbial detection in complex biological samples. By eliminating the traditional nucleic acid extraction step, a streamlined workflow enables direct amplification from human urine while maintaining strong sensitivity and reliability. Using a broad-range master mix, researchers can move from sample collection to results more quickly, reducing turnaround time by up to two hours and lowering per-sample costs. Comparative data across multiple UTI-associated bacterial targets show that extraction-free amplification can match—or in many cases outperform—results generated from extracted samples and synthetic controls. This approach supports efficient multiplexing, improved pathogen discrimination, and faster insights, making it a compelling alternative for high-throughput or time-sensitive workflows.

Gain insight into how simplifying sample prep could accelerate your next assay.