Employing An icIEF-UV/MS Workflow For Reproducible Separation Of NISTmAb Variants

By Rashmi Madda, Rita Nichiporuk, Mariam ElNaggar, Scott Mack, Maggie Ostrowski and Zoe Zhang SCIEX, USA

The Intabio ZT system offers direct chip-based integration of icIEF-UV with ZenoTOF 7600 system that can deliver rapid and reproducible separation and characterization of intact monoclonal antibodies (mAbs) charge variants, and assessment of proteoforms. In this study the charge variants of NISTmAb were monitored across 45 injections using 3 different cartridges to evaluate the reproducibility of the icIEF-UV/MS workflow.

Review data that supports the reproducibility of a streamlined icIEF-UV/MS workflow for charge variant separation, UV quantitation, and peak identification of mAbs by Intabio ZT system coupled to ZenoTOF 7600 system.