By Jason Brown, Process Development Engineer, Research and Development, and Nephi Jones, Senior Manager, Research and Development; Thermo Fisher Scientific
In moving processes from bench to pilot and ultimately production fermentors, it is critical to have a reliable scaledown model. The purpose of a scale-down model is to demonstrate at the benchtop level what can be achieved in production-scale fermentors. Scale-down models can vary depending on the media, strain, and process conditions. In this case, a customer wanted a scale-down model of the Thermo Scientific™ HyPerforma™ Single-Use Fermentor (S.U.F.) in order to establish what fermentation processes could be scaled into the S.U.F., in particular for the production of plasmid DNA. The 300 L S.U.F. exceeded the customer’s expectations. The customer anticipated that the test process using E. coli with high oxygen consumption would reach a final OD of ~80 and the HyPerforma S.U.F. exceeded expectations by reaching an OD of 147.