Enhanced Development Of Virus-Specific Hybridomas Using ClonePix And CloneSelect Imager Technologies

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Double-stranded DNA (ds-DNA) viruses are responsible for a wide range of diseases in humans. Notably, species within the order Herpesvirus and the families Adenovirus and Papillomavirus can cause similar symptoms, which makes diagnosis challenging. The difficulty in distinguishing these viruses arises from the conservation of antigens within their genomes, leading to high proteomic homology and serum cross-reactivities. This results in poor differentiation between individual ds-DNA virus species using traditional serological and protein-based diagnostic methods.

The application note aims to identify an optimal producer cell line capable of secreting a highly specific, non-cross-reactive monoclonal antibody for use in biotherapeutics development. To achieve this, the ClonePix™ System was utilized for high-throughput screening of hundreds of sub-cloned colonies derived from parental hybridoma material. Viable clones with high IgG secretion were further assessed for growth characteristics using CloneSelect™ Imager technology. This highly efficient automated clone selection process facilitated the rescue and stabilization of a high-titer hybridoma cell line that produces a highly specific antibody targeting an immunogenic viral antigen. The combination of these technologies provided sufficient material for the development of immunodiagnostic devices.

Access the full application note to learn more.