It is important to continue product improvement to enable the achievement of higher cell mass or product titer in less space. As users of HyPerforma S.U.F.s continually push to increase production levels in their fermentors, we continue to increase our product capabilities to meet their needs. We have enhanced the HyPerforma S.U.F. to meet higher cooling requirements of exponentially growing, high oxygen–consuming cultures. In this paper we examine how by enlarging the jacketed surface area we were able to increase cooling capacity of the reused support vessel. Also developed was a larger single-use parabolic turbine impellers with greater oxygen delivery capabilities and lower power consumption than equivalently scaled Rushton impellers on the original HyPerforma S.U.F.