Application Note

Growth And Scale-Up Of HEK293F Derivatives In HyPerforma DynaDrive Single-Use Bioreactors

By Paula Decaria, Senior Engineer, Research and Development, Thermo Fisher Scientific

dynadrive.jpg

The goal of these studies was to evaluate the performance of HyPerforma DynaDrive S.U.B.s for growth and scalability from 50 L to 3,000 L scales using two different HEK293F cells lines. These experiments were designed to demonstrate that HyPerforma DynaDrive S.U.B.s can be successfully implemented for use with HEK293 cell lines at increasing volumes with standard scale-up criteria. The first cell line was tested at multiple volumes within the 50 L S.U.B. to demonstrate in-vessel scale-up that would be typical for a transient transfection process. The second cell line was first tested at multiple volumes within the 50 L vessel, and then scaled from our 50 L to our 3,000 L vessels to demonstrate scalability of the systems.

VIEW THE APPLICATION NOTE!
Signing up provides unlimited access to:
Signing up provides unlimited access to:
  • Trend and Leadership Articles
  • Case Studies
  • Extensive Product Database
  • Premium Content
HELLO. PLEASE LOG IN. X

Not yet a member of Pharmaceutical Online? Register today.

ACCOUNT SIGN UP X
Please fill in your account details
Login Information
ACCOUNT SIGN UP

Subscriptions

Sign up for the newsletter that brings you the industry's latest news, technologies, trends and products.

You might also want to: