Growth And Scale-Up Of HEK293F Derivatives In Single-Use Bioreactors

By Paula Decaria, Senior Engineer, Research and Development, Thermo Fisher Scientific

The goal of these studies was to evaluate the performance of DynaDrive S.U.B.s for growth and scalability from 50 L to 3,000 L scales using two different HEK293F cells lines. These experiments were designed to demonstrate that DynaDrive S.U.B.s can be successfully implemented for use with HEK293 cell lines at increasing volumes with standard scale-up criteria. The first cell line was tested at multiple volumes within the 50 L S.U.B. to demonstrate in-vessel scale-up that would be typical for a transient transfection process. The second cell line was first tested at multiple volumes within the 50 L vessel, and then scaled from our 50 L to our 3,000 L vessels to demonstrate scalability of the systems.