Article | August 24, 2020

Guidance For Optimization Of A Real‐Time qPCR Assay

By Sarah Teter, Ph.D., and Leta Steffen, PhD, Promega Corporation

Scientist

Optimizing the formulation of reagents for your qPCR assay requires careful experimental design that looks across several performance factors for each reagent formulation tested. These performance factors include assay specificity, reliability, repeatability, linearity, sensitivity and efficiency.

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