How Much Media, PBS And Trypsin Do I Use With My Nunc™ Cell Factory™ System?

Culturing in a Thermo Scientific™ Nunc™ Cell Factory™ system is as simple as culturing in T-flask or dish. Seed the Cell Factory system with the same medium and cell density (cells/cm2) as in a flask or dish. A volume of 150-200 mL per tray (2.4 - 3.2mm depth) is commonly used. Simply identify the size of the Cell Factory system you are using in the chart below and apply the suggested working volumes for each step of the cell culturing process."