HPLC Autosampler Performance I: Challenging USP Methods On The Alliance™ iS HPLC System

By Norris Wong, Corey Reed, Amanda B. Dlugasch, Paula Hong

Autosampler performance in High-Performance Liquid Chromatography (HPLC) systems can be significantly affected by challenging method conditions. Methods involving highly organic sample diluents and low injection volumes often compromise injection precision, potentially leading to failed system suitability tests. To assess this impact, we evaluated the injection precision of an Alliance iS HPLC System using four USP methods known for their demanding conditions. Peak area reproducibility served as a proxy for autosampler performance.

Our study subjected the Alliance iS HPLC System to a rigorous three-day continuous run, simulating the demanding conditions of a high-throughput laboratory. Despite the challenging USP methods, the system consistently demonstrated exceptional precision, meeting all required system suitability criteria.

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