Hydrophilic Interaction Liquid Chromatography (HILIC) Method Migration Part 1: From Legacy HPLC Systems To The Alliance™ iS HPLC System

By Elom Y Pedanou, Paula Hong, Neil J. Lander

This application note helps illustrate a successful hydrophilic interaction liquid chromatography (HILIC) method migration from legacy HPLC systems across industries to the Alliance iS HPLC System. Due to differences in HPLC instrument designs, HILIC method migration could be challenging since results could be impacted. A well-designed system helps overcome those challenges to allow for seamless migration. For this study, the United States Pharmacopeia (USP) monograph for cetirizine hydrochloride assay and organic impurities were analyzed on two legacy HPLC systems and migrated to the Alliance iS HPLC System. Evaluate the performance of the method as well as the results from the experiment.