Improved Chlorhexidine Carryover Performance Using The Alliance iS HPLC System

The common need to develop and run methods on a variety of HPLC platforms is ubiquitous across the pharmaceutical and biopharmaceutical industries. When migrating methods across different LC systems, it is crucial to obtain consistent method performance regardless of the LC system used.

Sample carryover is one method performance parameter that can often be overlooked or not well investigated, since critical method performance typically includes attributes such as retention time and area precision, peak resolution, and signal to noise. Carryover occurs when an analyte from one injection is seen in subsequent injections. This can happen due to the adsorptive properties of analytes causing them to stick somewhere in the flow path or because of void volumes contained in the flow path.

Carryover performance across various HPLC systems was assessed using a scaled method based on the USP monograph for chlorhexidine hydrochloride organic impurities.