In-Process Media Monitoring Using The BioAccord™ LC-MS System For The Automated High Throughput Multi-Parallel Ambr® 15 Microbioreactor System

By Yun W. Alelyunas, Charles Prochaska, Clint Kukla, Mark D. Wrona, and Ying Qing Yu

Cell culture media provides essential nutrients and components required to sustain cell health and growth and for maximizing biotherapeutic production and quality. There is increasing interest from bioprocess groups to monitor raw media, feed, supplements and spent cell culture media, while providing this information quickly to process engineers. In addition to essential amino acids, vitamins, and other primary feed components, spent media contains hundreds of additional compounds and metabolites.

Routine comprehensive monitoring could provide additional information about cell culture performance during clone selection and process optimization. For this application note, methodology developed for cell culture media monitoring based on the BioAccord™ LC-MS System¹ is applied to the routine screening of in-process samples from an Ambr ® 15 high throughput bioreactor system. Learn more about this comprehensive LC-MS methodology, workflow, and batch analysis software that allows engineers to quickly and easily run and process large number of samples and gain insight during bioprocess development.