Inside Bioprocessing: Continuous Perfusion

In this episode of the Inside Bioprocessing podcast, industry experts explore the operational realities of shifting from traditional batch methods to continuous perfusion bioprocessing. While perfusion has historically supported unstable proteins, innovations in single-use technology and automation are now driving its adoption for broader biopharmaceutical production. Implementing a successful workflow requires navigating complex variables, from selecting high-productivity cell clones to managing the logistics of significant liquid transfers.

Beyond the standard continuous approach, manufacturers must distinguish between methodologies like N-1 perfusion and intensified fed-batch to align with specific production goals. Success relies on rigorous process analytical technologies (PAT) and validating scaled-down models to ensure stability over extended run times. Tune into the full episode to gain strategies for optimizing media usage, reducing manufacturing footprints, and leveraging data modeling for efficient commercial-scale production.