Mass transfer in Thermo Scientific™ HyPerforma™ Single-Use Bioreactors (S.U.B.s) is critical to the growth of cells in culture, yet is often a misunderstood principle due to its complexity. A basic understanding of mass
transfer and its underlying principles is essential to single-use bioreactor design, determining bioreactor operating parameters, and optimizing culture conditions. This paper aims to elucidate the standard mass transfer model, and details how the application of critical mass transfer principles to the design and operation of the HyPerforma S.U.B. helps to achieve optimal mass transfer performance for cell culture operations.