Isothermal Titration Calorimetry In Organic Solvent Systems

Isothermal titration calorimetry (ITC) is a well-established tool to study the binding of macromolecules. Understanding the non-covalent forces that drive these interactions can lead to meaningful advances in the fields of life sciences, pharmaceutical sciences, and drug development.

ITC has recently received increased attention from the field of material science, which necessitates the use of non-aqueous solvent systems. Herein, learn about the development of a representative binding assay that measures the interactions of Ca2+ with a calcium ionophore in a methanol solvent system.