For large-scale applications, when greater than 12 L of microcarrier beads require separation, the 25 L or 50 L Harvestainer system is well-suited for in-process microcarrier separation workflows. This system consists of a 200 L 3D BioProcess Container with inner 25 L microbarrier 2D BioProcess Containers that fits into a conical bottom drum as the secondary containment device. The BioProcess Container features a dip tube design for better drainage and minimal manipulation.
- Bioprocess Container (BPC)—composed of three layers; the outer two layers are constructed of Therm Scientific™ CX5-14 film with the inner layer constructed of polyester mesh
- Microcarrier inlet line—for easy system connection, the inlet line is made of weldable 1/2 x 5/8 in. C-Flex™ tubing with a 1/2 in. quick-connect insert on the inlet line
- Drain line—for easy system connection, the drain line is made of weldable 1/2 x 5/8 in. C-Flex tubing with a 1/2 in. quick-connect body; the dip tube drain line is connected to a dipwell and placed in a conical bottom drum to allow for optimal drainage and secondary containment with the top drain feature
- Exhaust line—exhaust line to be used for inflation of the Harvestainer BPC for setup and allow for air displacement during the separation process