Application Note

Mass Propagation Of Nicotiana Tabacum Cv. BY‐2 Suspension Cells In The Finesse Smartrocker Bioreactor

Source: Thermo Fisher Scientific

This application note describes the mass propagation of Nicotiana tabacum cv. BY-2 (cultivar tobacco Bright Yellow-2) suspension cells in the Finesse SmartRocker bioreactor at laboratory scale. Maximum fresh cell weights of about 351 ± 9 g•L-1, corresponding to packed cell volumes of 74 ± 2%, were achieved, which shows the applicability of the Finesse SmartRocker bioreactor to cultivate fast growing plant suspension cells. By Nicole Imseng, Stephan C. Kaiser, Nadezda Perepelitsa, and Dieter Eibl