The Thermo Scientific™ 2,000 L HyPerforma™ Single-Use Mixer (S.U.M.) provides rapid mixing for large-scale upstream and downstream processes. The addition of the Touchscreen Console to the 2,000 L HyPerforma S.U.M. provides real-time sensor visualization, data logging, and the ability to automate user-defined recipe mixing. To demonstrate the superior mixing capabilities for various applications and to determine optimal mixing parameters, five different mixing studies were performed with the 2,000 L HyPerforma S.U.M. at various working volumes and mixing speeds. The materials used in the mixing studies were chosen to demonstrate the way buffers (NaCl solution), complex media (Gibco™ AGT™ medium), and viscous liquids (corn syrup) mixed in the 2,000 L HyPerforma S.U.M.