The 2,000 L Thermo Scientific™ imPULSE™ Single-Use Mixer (S.U.M.) provides innovative mixing for upstream and downstream processes. The unique design of the imPULSE S.U.M. produces more effective mixing at lower working volumes than other mixers currently on the market, and produces one-way flow mixing. The addition of the Touchscreen Console to the imPULSE S.U.M. adds real-time sensor visualization, data logging, and the ability to automate user-defined mixing recipes. To demonstrate the superior mixing capabilities for various applications and to determine optimal mixing parameters, four different mixing studies (Table 1) were performed with the 2,000 L imPULSE S.U.M. at various working volumes and mixing settings. The materials used in these studies were chosen to demonstrate the way buffers (NaCl solution), complex media (Gibco™ AGT™ medium), and viscous liquids (corn syrup) are mixed in the 2,000 L imPULSE S.U.M.