One-Step Isolation And Activation Of Naive And Early Memory T Cells With CTS Dynabeads CD3/CD28
Here we demonstrate nearly 100% recovery of naive and early memory T cells using Gibco™ CTS™ Dynabeads™ CD3/CD28 beads. This one-step process of simultaneous T cell isolation and activation yields pure and uniformly activated T cells. CD4+ and CD8+ T cells expanded with CTS Dynabeads CD3/CD28 maintain an early memory phenotype on day 10 and have increased clonal diversity. Early bead removal is feasible and improves transduction efficiency of a γ-retrovirus. CTS Dynabeads CD3/CD28 beads deliver reliable results and have been used in more than 80 clinical trials and in commercial T cell drug manufacturing. Blood collection
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