Part 1: Intuitive Bioprocess Scale-Up From Bench Scale To Pilot Scale

By Jace Parkinson, Engineer II, Systems Design, Thermo Fisher Scientific

Scaling up bioprocesses from bench scale to pilot scale is a crucial phase in bioproduction, presenting unique challenges due to the physical differences between systems. This application note details a comparative study on the performance of Thermo Scientific™ DynaDrive™ Single-Use Bioreactors (S.U.B.) in scaling up a CHO-based bioprocess from 3L glass bioreactors to 50L S.U.B.s.

The study evaluates control parameters and outcomes, demonstrating that the DynaDrive S.U.B. not only meets but often exceeds key performance indicators of the bench-scale bioreactor. Key metrics such as IgG titer, specific productivity, and cell density were closely monitored, showing that the scale-up process using the DynaDrive S.U.B. offers reliable and efficient transition to larger scales while maintaining or improving bioprocess performance.

This document provides a comprehensive overview of the methodologies, results, and implications for successful bioprocess scale-up.