Part 1: Replicating An HPLC Method For Water-Soluble Vitamins On The Alliance™ iS HPLC System

By Kimberly Martin, Lise Gauthier, Paula Hong

Given the growing demand for vitamin supplements, it's crucial to verify that these products contain the advertised amounts. High-performance liquid chromatography (HPLC) is a vital analytical technique for ensuring label accuracy. However, the diverse chemical properties of vitamins make it difficult to analyze them all using a single chromatographic method. For water-soluble vitamins, hydrophilic interaction chromatography (HILIC) offers effective retention of these highly polar compounds. Furthermore, the complex matrices in which these vitamins are found often necessitate time-consuming sample preparation.

In regulated laboratories, the ability to transfer analytical methods between different HPLC systems is essential as equipment is updated or replaced. This study aims to demonstrate the transferability of methods for water-soluble vitamins analyzed under gradient HILIC conditions across various HPLC systems. By comparing quantitative results obtained on both legacy and newer systems, we will highlight the consistency and reliability of this approach.