Application Note

Powerfully Simple Digital PCR

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Digital PCR (dPCR) is gaining popularity as a tool for the quantification of residual DNA as an impurity in gene therapy development and manufacturing. It's growing use can be attributed to higher sensitivity and independence of running standard curves, compared with standard qPCR.

E. coli host cells are commonly used to produce various therapeutic biologics. Evaluate the resDNASEQ™ Quantitative E. coli DNA Kit (Cat. No. 4458435) based on it's detection sensitivity, quantification accuracy, and replicate uniformity as it performed when tested on the Applied Biosystems™ QuantStudio™ Absolute Q™ Digital PCR System.

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