Reproducibility Study Of A High-Throughput Released N-Glycan Workflow

By Fang Wang and Marcia Santos

One demanding, and important, step of early biopharmaceutical development is the screening of several clones and cell culture expression conditions quickly and efficiently.

This demonstration showcases the BioPhase Fast Glycan Labeling and Analysis kit, which yielded 576 data points from 2 instruments, 2 cartridges and 3 chemistry kits during inter- and intracapillary reproducibility validation. Glycan expression profiling is a closely monitored critical quality attributes (CQA) during the screening process, as it plays a significant role in the stability, safety and efficacy of protein therapeutics. Explore the key features of the released N-linked glycan analysis and the robust, high throughput analytical characterization tool delivering reliable instrumentation and flexible data analysis software for large-scale sample analysis.