One key factor for ensuring comparable growth and productivity during cell culture scale-up is pH control. pH impacts many factors in this process, including specific productivity, cell growth, and cell metabolism. The buffering capacity of the medium and feed is important in controlling pH and therefore developing a scalable and reproducible process.
For this work, we leveraged a pH shift, continuous feeds of EfficientFeed C+ Supplement and glucose, and the parameters outlined in Table 1. This approach resulted in a reproducible lactate profile and enabled consistent cell growth and productivity in 5 L to 250 L bioreactors.