Scaling And Migration Of A Hydrophilic Interaction Liquid Chromatography (HILIC) Method For Related Compounds Of Ribavirin To Modern HPLC Systems

Analytical methods often developed on sub-2 µm particle columns can be challenging to transfer to QC laboratories equipped with higher-dispersion HPLCs. This is due to differences in system characteristics, such as dwell and extra-column volumes, which can lead to peak distortion and inconsistent results.

This study demonstrates the successful scaling and migration of a HILIC method for ribavirin analysis from a sub-2 µm column to larger particle sizes (2.5 and 3.5 µm) on different HPLC systems. The goal was to ensure equivalent chromatographic performance and assay results across these systems.

By carefully considering system characteristics and optimizing the method for each platform, we were able to successfully scale and migrate the HILIC method for ribavirin analysis. This demonstrates the feasibility of transferring methods between different HPLC systems while maintaining analytical integrity.