Sensitive RNA Fluorescent Quantitation With The Quant-iT RiboGreen RNA Assay Kit

By Beverly Pappas, PhD, Molecular Devices

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The accurate quantification of nucleic acid concentration is crucial for various downstream applications, such as transfection, cloning, PCR, and next-generation sequencing (NGS). These applications often require specific target nucleic acid concentrations to achieve optimal performance. Inaccurate quantification can lead to increased variability in downstream assays and negatively impact the quality of results. RNA is typically quantified using microplate readers by measuring absorbance at 260 nm. However, absorbance-based methods have several disadvantages, including interference from contaminants, signal contributions from nonspecific proteins and free nucleotides, and limited sensitivity.

The Quant-iT™ RiboGreen RNA Assay Kit from Thermo Fisher Scientific offers a more specific method for RNA quantification. In this application note, we demonstrate that the Quant-iT RiboGreen assay can reliably measure RNA concentrations as low as 100 pg/mL using the SpectraMax® iD5 Multi-Mode Microplate Reader.

Access the full application note to learn more.