Tools To Assist In Moving Established Methods From An Originator HPLC System To An Alliance™ iS HPLC System

By Kaveh Amini Paula Hong

Transferring methods between HPLC systems can be a complex process due to differences in system characteristics. Retention time shifts often occur due to variations in dwell volume and gradient delay. Additionally, manual method creation and copying can introduce errors. This article explores how embedded software tools can significantly reduce the risk of errors and streamline the method transfer process to the Alliance iS HPLC System.

The Intelligent Method Translator App (iMTA) is a powerful tool that automates the transcription of critical method parameters from one HPLC system to another. By accurately transferring these settings, the iMTA helps ensure consistent method performance on the Alliance iS.

Furthermore, the Gradient Smart Start feature addresses the challenge of gradient delay variations between systems. By automatically adjusting the gradient start time relative to injection, this tool eliminates the need for manual adjustments to the gradient table, further simplifying the method transfer process and minimizing the risk of errors.

This example demonstrates the benefits of utilizing these embedded software tools in method replication or transfer. By automating key tasks and reducing manual intervention, these tools enhance efficiency, accuracy, and compliance, ultimately streamlining the transition to the Alliance iS HPLC System.