Bio-Techne Application Notes
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Automated Particle Analysis Of Viscous Samples With MFI And The Bot1 Autosampler
2/19/2021
Micro-Flow Imaging™ (MFI) easily detects particle size and morphology on a wide range of particle contaminants. And when you add the Bot 1 Autosampler to your MFI 5000 Series system, you’ve got the automated go-to method of choice for particle analysis today. In this application note, we studied how the Bot1 handled viscous samples, and found that with just a few simple optimization steps, you can be running viscous samples in no time at all.
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Protein Expression Heterogeneity With Milo, The First Single-Cell Western System
2/18/2021
Every cell is unique, making cell-to-cell heterogeneity important in many areas of biomedicine including cancer pathogenesis, immuno-oncology and regenerative medicine. More and more high-profile publications are using single-cell analysis techniques to reveal variability in cellular response to a drug or stimulus. Milo, the first and only Single-Cell Western platform out there, lets you do Western blotting at the single-cell level.
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A Custom, Multiplex Human Coronavirus Serological Assay
2/18/2021
R&D Systems, a Bio-Techne brand, has developed a novel custom Luminex serological assay hat distinguishes between coronavirus subtypes. Using our high-quality recombinant proteins, this antigen-down assay saves time and precious sample by multiplexing many coronavirus subtypes simultaneously in each sample.
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Wes And Milo Synergize To Profile Immune Cell Populations In The Tumor Microenvironment
2/4/2021
The population of patients who respond to immunotherapy are a minority. Accumulating evidence suggests that the heterogeneous makeup of the tumor microenvironment (TME) is a major contributor to the discrepancies in response observed. Profiling the composition and function of immune cells that exist within the TME is poised to guide an improved response to immunotherapy and may uncover novel therapeutic targets and strategies. In this application note, we’ll show you how Wes™ and Milo™ partner to get you critical answers to what type of immune cell populations are present in a sample and then what percentage of cells in that sample make up a specific immune cell subtype.
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Reprobe Your Immunoassay Samples Using RePlex With Jess
2/4/2021
The identification and quantification of protein expression levels is critical to understanding biological processes like those that drive cancer and other diseases. Unfortunately, limited samples sizes are far too common in these research areas, leaving researchers desiring more data from their limited samples. In this application note, we show how you can use RePlex to detect and quantify pan and phospho isoforms of proteins such as those in the PI3K/Akt signaling pathway.
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A Multi-Antigen Serology Assay For COVID-19 Using Simple Western
2/4/2021
The characterization of the human immune response to SARSCoV-2 is central to understanding COVID-19 disease progression and the efficacy of a vaccine. In this application note, we show how the SARS-CoV-2 Multi-Antigen Serology Module for Jess/Wes from ProteinSimple allows for simultaneous detection of human serum IgG reactivity with 5 viral antigens commonly associated with COVID-19. This kit provides a richer view into the immune response to SARS-CoV-2 in a single, quick, and easy assay.
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Learn How Fully Automated Westerns Enable Cutting-Edge Targeted Protein Degradation Research
2/2/2021
To characterize the efficacy of Degrader molecules, researchers generally run dose response curves by way of traditional SDS-PAGE Western blotting methods. But the lengthy, manual workflow and resulting low reproducibility make it an unreliable approach for the determination of DC50 values. Instead, the ideal solution would be highly reproducible, allow for easy quantitation and have a short time to results. Simple Westerns are just that—they let you separate and analyze proteins by size (or charge) from 2 kDa to 440 kDa in just 3 hours.
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Bioprocess Contaminant Detection Using Simple Western
2/2/2021
The identification and quantitation of process-related impurities during biotherapeutic development is critical to demonstrating the quality, efficacy and safety of the therapeutic agent. In this application note, we focus on the accurate detection of four candidate contaminants that may be present during various stages of the therapeutic protein and vaccine development processes: host cell protein (HCP), Protein A, green fluorescence protein (GFP) and bovine serum albumin (BSA).
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Automated Profiling Of PROTAC-Induced Cereblon Neosubstrate Degradation
2/2/2021
In this study, we demonstrate the time savings achieved by automating large screens and accurately quantifying DC50 and Dmax values for specific Degraders and IMiDs.
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Simple Western Analysis Of Adeno-Associated Virus (AAV) Proteins For Cell And Gene Therapy
2/1/2021
Western blotting is notoriously challenging; it’s labor-intensive, suffers from poor reproducibility, and is only semi-quantitative. For this reason, the Cell and Gene Therapy Catapult (CGTC), an independent center of excellence at the forefront of technology and innovation for cell and gene therapy commercialization, is adopting next generation technologies to advance quality control (QC) of AAV-mediated gene therapy. In this application note, you’ll see how CGTC has used highly-specific antibodies exclusively manufactured by PROGEN with fully automated Simple Western™ assays on Wes™ to monitor and characterize AAV capsids during product purification.