Bio-Techne Application Notes
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Fast And Reproducible AAV2 Quantification With Automated ELISA
7/30/2021
As part of the viral quantification process, researchers rely on various analytical methods to assess critical quality attributes. While traditional plate-based and dot blot assays can be useful, they are often complex, time-consuming, and non-standardized. In this application note we demonstrate how AAV2 capsid titer quantification with the Simple Plex AAV2 assay is a robust alternative to the conventional ELISA or dot blot approaches.
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Assess The Purity Of Your Cell Therapy Product With Confidence
6/4/2021
The analysis of particle contaminants in cell therapy products is critical for ensuring drug purity and safety. However, the successful characterization of such particulates requires gaining morphological insights into each type of particle present. A technique called micro-flow imaging (MFI) enables the visualization of particulate matter by providing relevant morphological details for further study of each type of particle identified. In this study, MFI was used to distinguish between NK cells and its expansion component, Cloudz™ microspheres, in different environments.
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Advance Your Western Workflow Into The 21st Century With Abby
5/18/2021
Protein analysis comes with many challenges, typically requiring labor-intensive, time-consuming protocols like the Western blot, which has multiple hands-on steps that increase user error and data variability. Abby™ is the latest Simple Western™ instrument dedicated to changing the way scientists analyze proteins by eliminating common protein analysis workflow challenges. Here we show how Abby delivers picogram-level sensitivity, while the RePlex assay enables detection of multiple targets per sample as well as total protein detection.
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Concentrating On AAV Impurities With Ultrasensitive Total Protein Detection On Simple Western
4/6/2021
Impurities in protein products can be dangerous and impact efficacy. For example, protein impurities in final drug products could lead to undesirable immune responses in patients, so detecting total protein is critical for revealing impurities in preparative protein production. Simple Western™ assays on instruments like Jess and Wes from ProteinSimple offer fully automated protein separation and quantification with small sample volumes, and sensitive chemiluminescent-based immunodetection and total protein detection.
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Using Avi-Tag Biotinylated Proteins To Determine Protein Interaction Kinetics And Affinity With Surface Plasmon Resonance
3/2/2021
Surface plasmon resonance (SPR) is a powerful, accurate, and direct way to measure protein interactions in real-time without the need for antibodies or detection reagents. In this study, we employ R&D Systems Avi-tag Biotinylated Proteins along with streptavidin Series S chips to specifically and uniformly capture proteins in a Biacore experiment, as an alternative to chemical or amine coupling.
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Monitoring Target Engagement In Drug Discovery: Application Of Wes To The Cellular Thermal Shift Assay
3/2/2021
In drug discovery, confirmation of in-cell target engagement is a critical component of the drug development process. In this application note, quantitative and reproducible CETSA data generated with ProteinSimple’s Wes™ instrument (CETSA-Wes) are presented. This assay verifies drug–target binding, and given its quantitative nature, the half maximal inhibitory concentration (IC50) is also calculated.
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Preclinical CAR-T Cell Target Safety, Biodistribution, And Tumor Infiltration Analysis Using The RNAscope® ISH Assay
3/1/2021
In this report we illustrate a way to detect activated CAR-T cells in the tissue context, as well as low expression of CAR target antigen, using the RNAscope® in situ hybridization (ISH) technology.
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Automated, Rapid, Reproducible Quantification Of HIV-1 Gag p24
2/22/2021
Reliable methods for quantification of Lentiviral vectors support process development and enables quality control of both vector and cell manufacturing. Traditionally ELISA assays serve as a common methodology for calculating viral titres and are used to measure the amount of viral capsid p24 protein in the supernatant of packaging cell lines. The 3.5 to 6 hours required to run a Standard ELISA is a challenge for operators who who increasingly require results quickly to inform real time decision making. The Simple Plex p24 assay, hosted on Ella - an automated immunoassay system, can rapidly quantify p24 levels expressed from lentiviral vectors during different stages of cell and vector manufacturing.
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A Fixation Protocol Compatible With R&D Systems Luminex Assays Enabling Analysis With Infectious Samples
2/22/2021
A common way to inactivate infectious material is to use formaldehyde fixation. Confirmation of microorganism inactivation is accomplished using viability testing with broth or growth media for bacteria and plaque assays for viruses. Unfortunately, chemical fixation using formaldehyde has been shown to alter cytokines and chemokines in such a way that an immunoassay would not properly quantify biological samples. To address this issue, we decided to test the hypothesis that R&D Systems Luminex Assays are compatible with standard fixation protocols.
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Residual Protein A For Bioprocessing Applications
2/19/2021
Most therapeutic monoclonal antibodies (mAbs) are isolated from cell culture media using Protein A chromatography. During purification, Protein A can leach from the solid phase, ending up in the purified product. This can be problematic as regulatory guidelines dictate that detection and reduction of residual Protein A are necessary to obviate potential immunogenic consequences in vivo. ProteinSimple’s Wes™ provides a powerful solution by bringing immunoassay into a capillary electrophoresis format.