Cytiva product datasheets, catalogs, app notes

31. Scale-Up Of CHO Cell Fed-Batch Cultures 2/21/2020

    This application note compares cell growth and recombinant protein production of multiple CHO cell clones when cultured in HyClone ActiPro basal medium and Cell Boost 7a and 7b feeding supplements.

32. Effects Of Feeding Strategy On CHO Cell Performance In Fed-Batch Cultures 2/21/2020

    Here, we demonstrate a fed-batch process for a CHO cell line producing a mAb. The results emphasize the importance of an appropriate feeding strategy to optimize cell growth and productivity.

33. Purifying Tagged Proteins Using A Small And Compact LC System 2/3/2020

    Learn how you can perform routine protein purification in a variety of common applications with ease while efficiently utilizing bench and cold cabinet space with the ÄKTA go.

34. Epitope Binning: A Critical Step In Antibody Selection 1/31/2020

    Epitope binning immunoassays using surface plasmon resonance (SPR) can and should be deployed in the development of new potential drug candidates. This article discusses how SPR is used to characterize closely related “bins” of mAbs targeting the same epitope of the target protein and how the selection process is used to develop new vaccines, therapeutic antibodies, and diagnostic tools.

35. An End-To-End, Semi-Continuous Process For mAb Production 9/22/2019

    See how the different unit operations in a laboratory-scale monoclonal antibody (mAb) process can be connected and integrated into a semi-continuous process.

36. Quantitating Binding Similarities In IgG Fcγ Receptor Analysis 5/23/2019

    Critical quality attributes are fundamental to regulatory compliance in drug development and manufacturing. Learn how surface plasmon resonance has been applied for protein characterization of antibody effector function.

37. Adenovirus Production In Single-Use Bioreactor System 3/8/2019

    Rocking bioreactor systems can shorten the seed train prior to inoculation and provide optimized growth conditions for sensitive cells. This study presents a robust production of adenovirus using such a system.

38. Multidimensional Scale-Up Of A mAb Capture Step Contributes To Shortened Time To Market 10/10/2018

    This application note demonstrates a multidimensional scale-up (change of both column diameter and bed height) of a mAb capture step, using the ÄKTA pilot 600 chromatography system.

39. Process-Economy Simulation Of mAb Capture Step With Protein A Chromatography Resin 10/10/2018

    Calculation examples that highlight scenarios where the enhanced capacity and alkaline‑stability of MabSelect PrismA can provide process economic benefits to large‑scale mAb processing.

40. Use Of Sodium Hydroxide For Cleaning And Sanitization Of Chromatography Resins And Systems 10/2/2018

    This application note examines these aspects of using sodium hydroxide as a cleaning and sanitizing agent.