Application Notes & Case Studies

1. Mixing Efficiencies For The 200L HyPerforma Single-Use Mixer 10/19/2021

   The Thermo Scientific™ 200 L HyPerforma™ Single-Use Mixer (S.U.M.) provides rapid mixing for essential upstream and downstream processes. The addition of the Touchscreen Console to the 200 L HyPerforma S.U.M. adds real-time sensor visualization, data logging, and the ability to automate user-defined mixing recipes.

2. Mixing Efficiencies For The 50 L HyPerforma Single-Use Mixer 10/19/2021

   Here we review the mixing capabilities for various applications and determine optimal mixing parameters using four different mixing studies using the 50 L HyPerforma S.U.M. at various working volumes and mixing speeds.

3. Production Of AAV Vector In HyPerforma S.U.B.s Using The ExpiSf Expression System 10/19/2021

   The ExpiSf Expression System is a scalable option for baculovirus processes for research use; it allows for high titers that are comparable to those typically seen in glass reactors.

4. HyPerforma DynaDrive S.U.B. For Perfusion Cell Culture Applications 10/19/2021

   With more biomanufacturing production processes moving toward perfusion, it becomes important to investigate new technologies to support processes that may stretch the limits of biology and engineering in legacy single-use systems.

5. Scale-Up Evaluation Of The HyPerforma DynaDrive S.U.B.s Part 1: ExpiCHO-S Cells And CHO-S Cells (cGMP banked) 10/19/2021

   Recently developed process intensification has allowed manufacturers increased product output. These output achievements require increased production efficiency and input, pushing many bioreactor systems past their limits. S.U.B. quality requirements, robustness, and functional performance can all become constraints. An effective single-use bioreactor must be able to scale up their process with minimal process changes to meet demand for commercial therapeutics.

6. Streamlining Seed Train Scale-Up Through Utilization Of The Turndown Ratio Of The HyPerforma DynaDrive S.U.B. 10/19/2021

   Enhancements to single-use bioreactor platforms have allowed for higher turndown ratios and optimized mixing and gassing strategies for intensified processes. These higher turndown ratios open a new paradigm of what is possible with seed trains, potentially eliminating multiple vessels and reducing logistical and operating costs dramatically while increasing the efficiency of the seed train through reduced connections and transfer losses.

7. Improved Viable Particle Discrimination With Real-Time Viable Particle Counter 10/18/2021

   A new algorithm developed for use by the BioTrak® Real-Time Viable Particle Counter determines the viability of a particle based on its measured optical properties. The new algorithm significantly improves the detection of microorganisms, most markedly molds.

8. Cell Line Development: Accelerating Antibody Discovery With The Octet® Platform 10/5/2021

   Cell line development involves multiple processes. Large numbers of clones are screened and selected on the basis of productivity and stability. An established platform for rapid titer of antibody clones can enable quick selection of high-producing clones.

9. Simplifying CPV Validation And Consistency 10/4/2021

   A leading organization in small molecule therapies was facing a challenge in ensuring consistent CPV initiatives across their different manufacturing sites was maintaining a uniform standard of data in their manufacturing analytics. A software solution that was purpose-built for biopharmaceutical manufacturing provided a strong foundation to streamline the validation of manufacturing analytics for CPV and reporting.

10. Condensation Control In Cleanroom Environments 9/28/2021

    Condensation in clean manufacturing facilities can lead to contamination of the environment and compromise the quality of products being produced, so must be avoided. Therefore, the thickness of insulation can have a significant impact on the ability to prevent condensation build up.