MilliporeSigma Application Notes & White Papers

  1. Middle-Up Mass Analysis Of Protease Digested Cetuximab 10/24/2022

    Explore a complete reversed phase UHPLC-MS workflow, developed to simplify middle-up mass analysis of an immunoglobulin G antibody, from the antibody purification, proteolysis, and reduction to its UHPLC-MS analysis.

  2. UHPLC Analysis Of Vitamin D2 & D3 10/21/2022

    Here, we demonstrate the use of the Supel™ Carbon LC UHPLC column to baseline separate the vitamin D2 and D3 forms from the D2 and D3 epimers.

  3. Workflow For Antibody Aggregate And Fragment Analysis 9/27/2022

    Characterizing mAb aggregate and fragment formation during drug formulation and establishing a suitable quality control process is essential. Learn about a complete workflow for antibody aggregate and fragment quantification.

  4. Supel™ BioSPME 96-Pin Device Comparison To Rapid Equilibrium Dialysis Technique 4/9/2022

    Here, we review a technology that has been demonstrated to significantly reduce the time required for protein binding determination when compared with the rapid equilibrium dialysis method.

  5. LC-MS/MS Method To Analyze 20 Amino Acids 4/8/2022

    The wide-ranging polarities spanning across all 20 amino acids can make analysis challenging. Learn about an LC-MS/MS method that achieves this, without derivatization, utilizing a carbon LC column.

  6. Rapid Microbial Detection Of Contaminants In Water Samples 4/7/2022

    Various water samples were tested to assess the ability of the Milliflex® Quantum System to detect microbial contaminants quicker and to evaluate the reduction of time-to-final result compared to the compendial method.

  7. Rapid Microbial Contamination Detection In Ethanol, Gel-Based Disinfectants 4/7/2022

    Ethanol and gel-based disinfectants must undergo QC controls to ensure an acceptable bioburden level. Discover a sensitive method for the quantitative rapid detection of contaminants in filterable samples.

  8. Workflow For High-Throughput Glycoprofiling Of Rituximab 4/1/2022

    Proper characterization of therapeutic mAbs is essential for ensuring drug safety and efficacy. Discover a complete SEC-MS workflow, developed to enable rapid glycoprofiling of mAbs in cell culture supernatants.